Bump, open to any and all suggestions.

So you've verified that the microcontroller is getting the correct voltage on the correct pin.
Do you see any other analog inputs (ADC) doing anything in tunerstudio? Or are all of them not doing anything? (coolant temp, air temp, 02, etc)
It is uncommon in modern microelectronics and even more uncommon in automotive spec stuff to fry a single pin; but it can happen.
If you did somehow achieve this, and other inputs are working, why not jump your map signal to the spareadc input? (pin29? (manual doesn't line up with silkscreen), edit: or pin16/17 based on silkscreen).

If none of the inputs are working then you have either a bad microsquirt module, corrupted firmware, or something more complicated at the microcontroller chip environment level.

I have the coolant temp, air temp, wideband, and vTPS all working. Lets say I did jump MAP to pin 29, how do I get TunerStudio to know this? Im guessing its a ini file change? Thats a pretty good idea but I would like to use that as a last resort.

Interesting. You'll need to take a good look at the silkscreen and make sure I got the pin right. The manual says pin29 but your silkscreen pic says pin16/17. I'd probably trust the silkscreen.
I actually have an ms3 so I'm not too sure about the differences in tunerstudio and the ms2.
For the ms3 there's a simple drop down menu under general settings that you can use to choose what ADC input the map signal is on.
I haven't messed with the ini files enough to know if you can tinker with them directly and have the java script still read it correctly.

That error about the MAF is interesting? You don't have the thing setup to use a mass air flow sensor do you?
Or have something odd in the ini file that the tunerstudio scripts cant read?

As others have said this sounds like setting issue.

Im pretty confident I got the right pin on the CPU. I mean I watched the voltage change when I sucked/blowed on the vacuum line. Ill feel really dump if its a settings issue. I swear I have went through every setting 10 times, but with that said I didnt see a drop down to choose the ADC input. Even if I was setup to use a mass air flow sensor, wouldn't the MAP sensor still register and change? Or just show 0 kPa?

Indeed, your map sensor is fine and its signal is getting to your microcontroller.
Now you need to tell the software that you're using the microcontroller pin that you've got physically connected to the map sensor.

I didn't write the software, but if I did, if you were to tell the system to use maf it wouldn't do anything with the map readout in the gui.
Maybe it shows the barometric value at startup or something. Maybe if you suck on the line and turn on the key it'll show something else haha.

Check your settings, make sure you're using speed density and your map adc input is correct.

From Googling, it looks like it is possible to move it over to a different pin but not as easy as MS3. This absolutely sucks. I will double check that I am using speed density. Thank you for your suggestions.

Also, in a perfect world this shouldn't matter, but I have been attempting all of this on a 64 bit computer running Ubuntu.

Tuner studio uses java and is pretty compliant across OS's.
Check out pages 67 and 71 of the manual. It has actual screenshots of the dropdown menus for the settings I've talked about.
Control algorithm should be set to speed density

If you're really confused post up pics of what you see.
http://www.msextra.com/doc/pdf/Megas...erence-3.4.pdf

This should be your bedtime story

also make sure the sensor calibration is correct.

Ok, I believe I have figured out the problem. I had to change the MAP sample method from the default of 'Use average' to 'Use lowest value'. Now Engine MAP changes when I suck/blow. Is there any reason why I shouldn't use lowest value? Otherwise Im gonna shout 3 "Hail Mary"s and start tuning this bitch. Thanks for all the help and input, I really appreciate it. Throughout this whole troubleshooting process I have learned so much about MegaSquirt and TunerStudio and how they work.

Never used that before. Something still sounds really wrong if you need that in order to get a reading.

Yeah, I agree. At the same time it has been running really really well so far so until I run into a problem I plan on just leaving it as is.